You will need: a U-100 insulin syringe, bacteriostatic water, and alcohol swabs.
Before you start: allow the bacteriostatic water to reach room temperature. Do not use it cold — cold water dissolves the lyophilate more slowly and less completely. Never warm or heat the water, as heat degrades the peptide.
- Swab the stopper of both the bacteriostatic water vial and the product vial with alcohol.
- Draw your target volume of bacteriostatic water into the insulin syringe. If your volume is more than 1 mL, use two or more draws — the syringe holds 1 mL.
- Insert the needle into the product vial and inject the water slowly, aiming the tip at the inner glass wall so it runs down the side — not directly onto the powder.
- Swirl gently until fully dissolved and clear. Do not shake (shaking foams and stresses the peptide).
- Once the solution is completely clear, draw it back into the U-100 syringe in precise unit increments for accurate aliquoting.
- Refrigerate the reconstituted vial (2–8 °C).

For laboratory research use only. Not for human or veterinary use, ingestion, or administration. This content is illustrative, not to scale, and provided for general educational purposes only — it is not medical or professional advice, and it is not instructions for use of any substance in or on the body of any human or animal.